THE  Point prevalence of acetylcholinesterase inhibition, neuropathy and safety awareness among flower farm workers in naivasha, nakuru county, kenya.

Scholastica Mathenge; Patroba Ojola; Hannah Wanjiru Mwangi

doi:10.37022/tjmdr.v4i1.553

Scholastica Mathenge Department of Medical Laboratory Sciences, Kenyatta University, Nairobi, Kenya
Patroba Ojola Department of Medical Laboratory Sciences, Kenyatta University, Nairobi, Kenya
Hannah Wanjiru Mwangi Department of Microbiology and Biotechnology, Kenyatta University, Nairobi, Kenya

DOI https://doi.org/10.37022/tjmdr.v4i1.553

Abstract

Acetyl cholinesterase catalyses the hydrolysis of acetylcholine in the nerve synapses, thereby terminating nerve impulse, however, it is inhibited by organophosphates and carbamates. This study aimed to assess the level of acetyl cholinesterase inhibition and the resultant neuropathy in flower farm workers as well as the farmers’ awareness of safety measures and predisposing factors while handling pesticides. A cross-sectional study was conducted involving 217 participants from different flower farms. Structured questionnaire was used to collect data to assess level of safety and predisposing awareness and to assess for neuropathy. Blood samples were collected to determine the cholinesterase levels using spectrophotometry technique at 405 nm. The mean serum cholinesterase level in flower farm workers in Naivasha was 5873.26 U/L. There was a positive correlation (R= 0.07) between the numbers of years worked in the industry and serum cholinesterase levels as shown by Karl Pearson`s coefficient. The point prevalence of cholinesterase inhibition in Naivasha was 14% indicating a significant risk of adverse health effects. The level of safety awareness and predisposing factors among workers on pesticide exposure was 65%, suggesting that while the majority of workers had some level of safety awareness, there is still room for improvement. The point prevalence of neuropathy was 38% of the participants presenting with varying symptoms of adverse effects of pesticide exposure. Musculoskeletal impairment was leading at 19.81%, followed by skin irritation at 13.36%.These findings highlighted the need for better safety measures and awareness campaigns in the flower farm industry to reduce the risk of acetyl cholinesterase inhibition.

Keywords: Neurotoxicity, acetyl cholinesterase, inhibition, safety and predisposing factor, pesticide

References

1. Tsimbiri, P. F., Moturi, W. N., Sawe, J., Henley, P., & Bend, J. R. (2015). Health impact of pesticides on residents and horticultural workers in the Lake Naivasha Region, Kenya. Occupational Diseases and Environmental Medicine, 3(02),
2. https://kenyanews.co.ke/flower-farming-in-naivasha-kenya/ kingatmo 2015
3. Shentema, M. G., Kumie, A., Bråtveit, M., Deressa, W., Ngowi, A. V., & Moen, B. E. (2020). Pesticide use and serum acetylcholinesterase levels among flower farm workers in Ethiopia—a cross-sectional study. International Journal of Environmental Research and Public Health, 17(3), 964.
4. Gupta, R. C. (2020). Neurotoxicity of organophosphate nerve agents. In Advances in Neurotoxicology (Vol. 4, pp. 79-112). Academic Press.
5. Calaf, G. M., Bleak, T. C., & Roy, D. (2021). Signs of carcinogenicity induced by parathion, malathion, and estrogen in human breast epithelial cells. Oncology Reports, 45(4), 1-1.
6. WHO. International Code of Conduct on the Distribution and Use of Pesticides: Guidelines for the Registration of Pesticides. World Health Organization; Rome, Italy: 2010. [Google Scholar]
7. Anode, S. O., Onguso, J., & Magoma, G. (2018). Qualitative and quantitative analysis of pesticide residues in flower farms soils around Lake Naivasha basin, Kenya. International Journal of Conservation Science (IJCS), 6(6), 1615-1623.
8. Mrema, E. J., Rubino, F. M., Brambilla, G., Moretto, A., Tsatsakis, A. M., & Colosio, C. (2013). Persistent organochlorinated pesticides and mechanisms of their toxicity. Toxicology, 307, 74-88.
9. Mwabulambo, S. G., Mrema, E. J., Ngowi, A. V., & Mamuya, S. (2018). Health Symptoms Associated with Pesticides Exposure among Flower and Onion Pesticide Applicators in Arusha Region. Annals of Global Health, 84(3), 369–379. DOI: http://doi.org/10.29024/aogh.2303
10. Kamel, F., et al. (2014). Neurobehavioral performance and agricultural exposure: A study among pesticide applicators in Egypt. Environmental Health 13(1):
11. Mirzaei, R., et al. (2019). Assessment of cholinesterase and paraoxonase activities in greenhouse workers exposed to pesticides. Journal of Environmental Health Science and Engineering 17(1): 55-61.
12. Tsakiris, S., et al. (2011). Reversibility of chlorpyrifos-induced inhibition of brain and erythrocyte acetylcholinesterase activity in rats. Toxicology Mechanisms and Methods 21(1): 43-49.
13. Delgado, E., Espinosa, M., Bassante, M., & Haro, V. (2015). Organophosphate pesticide exposure decreases serum butyrylcholinesterase activity in Ecuadorian flower farm workers. Environmental health insights, 9, 31-35.
14. Kumar, N., Sharma, A. K., Singh, A. K., & Srivastava, A. K. (2018). A comparative study of the effect of organophosphate pesticides on serum butyrylcholinesterase and alkaline phosphatase activity in Indian agricultural workers. Indian Journal of Occupational and Environmental Medicine, 22(1), 12-17.
15. Golbabaie, F., Dehghanifard, E., & Aminizadeh, M. (2020). Assessment of organophosphate pesticide exposure and cholinesterase activity among pesticide sprayers in a rural area of Iran. Human & experimental toxicology, 39(5), 559-567.
16. Fard, A. H., Kiani, F., Ghaffari, H. R., & Tarrahi, M. J. (2021). Organophosphate pesticide exposure and serum cholinesterase levels among agricultural workers in central Iran. Journal of environmental health science & engineering, 19(1), 371-377.
17. Ngowi, A. V., Maeda, D. N., Wesseling, C., & Partanen, T. J. (2014). Pesticide exposure and symptoms among smallholder farmers in the Lake Zone of Tanzania. Occupational and Environmental Medicine, 71(4), 254-261. doi: 10.1136/oemed-2013-101731
18. Jaga, K., & Dharmani, C. (2015). Sources of exposure to and public health implications of organophosphate pesticides. Revista Panamericana De Salud Pública, 38(3), 182-190.PubMed Indentifier: 26642363
19. Kwok, C. K., Leung, T. K., Chan, H. Y., Yiu, Y. M., & Wong, M. H. (2018). Pesticide safety training and practices among agricultural workers in rural China. Journal of Environmental Science and Health, Part B, 53(7), 452-458. doi: 10.1080/03601234.2018.1458961
20. Miri, A., Akbarzadeh, A., Mohammadi, H., & Tabatabaee, H. (2021). Effect of safety training on knowledge, attitude, and practice of farmers towards pesticide use. Journal of Environmental Health Science and Engineering, 19(1), 485-492. doi: 10.1007/s40201-021-00669-3
21. Hossain, M. S., Islam, R., Islam, M. S., Islam, M. A., & Biswas, S. K. (2019). Effect of safety training on pesticide handling practices and health outcomes of agricultural workers in Bangladesh. International Journal of Environmental Research and Public Health, 16(23), 4717. doi: 10.3390/ijerph16234717
22. Trang A, Khandhar PB. Physiology, Acetylcholinesterase. [Updated 2023 Jan 19]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK539735/
23. Z. Gabriel et al.Termination and beyond: acetyl cholinesterase as a modulator of synaptic transimission.Cell Tissue Research. (2006)
24. Yassin MM, Abu Mourad TA, Safi JM. Knowledge, attitude, practice, and toxicity symptoms associated with pesticide use among farm workers in the Gaza Strip. Occupation and Environmental Medicine. 2002; 59:387–93.
25. Sapbamrer R, Damrongsat A, Kongtan P. Health impact assessment of pesticide use among farmers from Phayao Province, Northern Thailand. Journal Environmental Research. 2011; 33:1–11.
26. Sapbamrer, R., & Nata, S. (2014). Health symptoms related to pesticide exposure and agricultural tasks among rice farmers from northern Thailand. Environmental health and preventive medicine, 19(1), 12-20.