Innovative niosomal targeted delivery system of crepidium accuminatum extract: evaluating anticancer potential in colon cancer ht-29 cell lines

saravana Raja; Jesindha Beyatircks; Karthik Annamalai; Lokeshwaran Lokeshwaran; Ciyasabu Ciyasabu; Divya Melepura

doi:10.46796/ijpc.v6i4.757

Saravanan Raja Assistant Professor, Department of Pharmacology, Hillside College of Pharmacy & Research Centre, Rajiv Gandhi University of Health Science, Bangalore-560102, India.
Jesindha Beyatircks HOD & Professor, Department of Pharmaceutics, Hillside College of Pharmacy & Research Centre, Rajiv Gandhi University of Health Science, Bangalore-560102, India.
Karthik Annamalai Assistant Professor, Department of Pharmacology, Kasthooribha Gandhi Pharmacy College, Masakalipatty Rasipuram, Namakkal, Tamil Nadu- 637401, India.
Lokeshwaran Assistant Professor, Department of Pharmaceutics, Hillside College of Pharmacy & Research Centre, Rajiv Gandhi University of Health Science, Bangalore-560102, India.
Ciyasabu Assistant Professor, Department of Pharmaceutics, Hillside College of Pharmacy & Research Centre, Rajiv Gandhi University of Health Science, Bangalore-560102, India.
Divya Melepura Associate Professor, Department of Pharmacology, Hillside College of Pharmacy & Research Centre, Rajiv Gandhi University of Health Science, Bangalore-560102, India.

DOI https://doi.org/10.46796/ijpc.v6i4.757

Abstract

Colon cancer remains a leading cause of mortality worldwide, necessitating innovative therapeutic strategies that enhance drug efficacy while minimising side effects. This study investigates the formulation and in vitro assessment of niosomes loaded with ethanolic extract from Crepidium acuminatum, a medicinal orchid known for its bioactive compounds. Niosomes were prepared using the thin-film hydration technique with Tween 60 and cholesterol in varying ratios to optimise encapsulation and release profiles. Physicochemical characterisation revealed high entrapment efficiency (up to 78.3% in the optimised F4 formulation), sustained drug release over 24 hours, and stability under refrigerated conditions. Fourier-transform infrared (FTIR) spectroscopy confirmed compatibility between the extract and excipients. In vitro cytotoxicity assays on HT-29 colon cancer cells demonstrated superior antiproliferative activity of the niosomal formulation compared to the free extract, with an IC50 value of 32.5 µg/mL versus 58.2 µg/mL. These findings underscore the potential of niosome-encapsulated C. acuminatum extract as a promising nanocarrier for targeted colorectal cancer therapy, warranting further in vivo validation.

Keywords: Niosomes, Crepidiumaccuminatum, Colon cancer, HT-29 cells, Drug delivery, In vitro cytotoxicity

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Author Biographies

Saravanan Raja, Assistant Professor, Department of Pharmacology, Hillside College of Pharmacy & Research Centre, Rajiv Gandhi University of Health Science, Bangalore-560102, India.

Assistant Professor, Department of Pharmacology, Hillside College of Pharmacy & Research Centre, Rajiv Gandhi University of Health Science, Bangalore-560102, India.

Jesindha Beyatircks, HOD & Professor, Department of Pharmaceutics, Hillside College of Pharmacy & Research Centre, Rajiv Gandhi University of Health Science, Bangalore-560102, India.

HOD & Professor, Department of Pharmaceutics, Hillside College of Pharmacy & Research Centre, Rajiv Gandhi University of Health Science, Bangalore-560102, India.

Karthik Annamalai, Assistant Professor, Department of Pharmacology, Kasthooribha Gandhi Pharmacy College, Masakalipatty Rasipuram, Namakkal, Tamil Nadu- 637401, India.

Assistant Professor, Department of Pharmacology, Kasthooribha Gandhi Pharmacy College, Masakalipatty Rasipuram, Namakkal, Tamil Nadu- 637401, India.

Lokeshwaran, Assistant Professor, Department of Pharmaceutics, Hillside College of Pharmacy & Research Centre, Rajiv Gandhi University of Health Science, Bangalore-560102, India.

Assistant Professor, Department of Pharmaceutics, Hillside College of Pharmacy & Research Centre, Rajiv Gandhi University of Health Science, Bangalore-560102, India.

Ciyasabu, Assistant Professor, Department of Pharmaceutics, Hillside College of Pharmacy & Research Centre, Rajiv Gandhi University of Health Science, Bangalore-560102, India.

Assistant Professor, Department of Pharmaceutics, Hillside College of Pharmacy & Research Centre, Rajiv Gandhi University of Health Science, Bangalore-560102, India.

Divya Melepura, Associate Professor, Department of Pharmacology, Hillside College of Pharmacy & Research Centre, Rajiv Gandhi University of Health Science, Bangalore-560102, India.

Associate Professor, Department of Pharmacology, Hillside College of Pharmacy & Research Centre, Rajiv Gandhi University of Health Science, Bangalore-560102, India.

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