Development and validation of new uv-visible spectrophotometric method for the estimation of minoxidil in bulk and pharmaceutical dosage form

Abstract

Minoxidil, an oral direct-acting peripheral vasodilator, lowers high systolic and diastolic blood pressure and is also applied topically to treat androgenetic alopecia. The present investigation is to develop a simple, precise and cost-effective UV method for method development and validation of Minoxidil in bulk and pharmaceutical dosage form. The calibration curve was constructed with concentrations ranging from 20 to 60 μg/ml. The correlation coefficients were found to be 0.9993. Accuracy method was ascertained by determination of the recovery of the method at three different concentrations by standard addition method. The results were within the range of 99.57– 100.55 % and were found to be highly accurate. The parameter LOD was determined on the basis of response and slope of the regression equation. The LOD for this method was found to be 5.08μg/ml. The parameter LOQ was determined on the basis of response and slope of the regression equation. The LOQ for this method was found to be 15.38μg/ml. The samples were tested and the results of ruggedness were between the ranges of 99.75 – 99.94 %. These values were found to be within the limits. In the present investigation, simple and sensitive UV spectrophotometric methods were developed for the quantitative estimation of Minoxidil in bulk drug and pharmaceutical formulations. In addition to positive requirements of these analytical methods, the striking advantages of all the presently developed methods were economical. The methods were validated in terms of linearity, accuracy, precision, ruggedness and robustness and used for the outline determination of Minoxidil in bulk drug and in pharmaceutical formulations.