A detailed study of phytochemical and invitro anticancer study on euphorbia heterophylla .l
Abstract
Health is the main issue of today’s life. Most the diseases are due to improper life style, Taking improper functional foods and by depend on fast, junk foods. Due to these foods, oxidation takes place and oxidation causes lysis of cell and it causes cancer Plants such as Euphorbia heterophylla are commonly found in our surroundings. They are also used for the management of different human diseases. The anticancer activities of leaves from these plants were investigated using aqueous extracts. The extracts are subjected to phytochemical analysis. The invitro methods were used for significant cytotoxicity on Human ovarian cancer cell lines, viz., Pa-1 cells with the IC50 concentrations (The Concentration of the Compound have the capacity to kill 50% of Viable Cells) at 57.08ug/ml respectively after the incubation period of 24hrs. The MTT assay is a colorimetric assay used for the determination of cell proliferation and cytotoxicity, based on reduction of the yellow coloured water soluble tetrazolium dye MTT to formazan crystals. The obtained results suggesting us that the EHE may have effective anticancer potential against the Human ovarian cancer (Pa-1) cells due to its low IC50 value.
Keywords:
phytochemical
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References
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3. Falodun A and Agbakwuru EO, 2004. Phyto-chemical analysis and laxative activity of the leaf extracts of Euphobia heterophylla Linn. (Euphorbiaceae). Pakistan Journal of Scientific and Industrial Research, 47(5):345-348.
4. Falodun A, Okunrobo LO and Uzoamaka N, 2006. Phytochemical screening and anti- in-flammatory evaluation of methanolic and aqueous extracts of Euphorbia heterophylla Linn (Euphorbiaceae). African Journal of Bio-technology, 5(6):529-531.
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7. Annapurna A and Hatware K, 2014. Effect of aqueous extract of Euphorbia heterophylla on blood glucose levels of alloxan induced dia-betic rats. International Journal of Research in Pharmacy and Chemistry, 4(3), 669-672.
8. James O and Friday ET, 2010. Phytochemical composition, bioactivity and wound healing potential of Euphorbia heterophylla (Euphor-biaceae) leaf extract. International Journal on Pharmaceutical and Biomedical Research, 1(1), 54-63.
9. Ughachukwu PO, Ezenyeaku CCT, Onwurah WO, Ifediata FE, Ogamba JO and Afonne OJ, 2013. Effect on some haematological indices of human whole blood when aqueous leaf extract of Euphorbia heterophylla was used as storage anticoagulant. African Journal of Biotechnolo-gy, 12(31), 4952-4955.
10. Kalaskar MG, Shah DR, Raja NM, Surana SJ, Gond NY, 2010. Pharmacognostic and Phyto-chemical Investigation of Ficus carica Linn. Ethnobotanical Leaflets, 14(5): 599-609.
11. Kalaskar MG and Surana SJ, 2012. Pharmacog-nostic and phytochemical studies on Ficus Mi-crocarpa L. fil. Ancient science of life, 32(2):107.
12. Nakata PA, 2003. Advances in our understand-ing of calcium oxalate crystal formation and function in plants. Plant Science, 164(6):901-909.
13. Neinhuis C, Barthlott W, 1997. Characteriza-tion and distribution of water-repellent, self-cleaning plant surfaces. Annals of botany, 79(6):667-677.
14. Metcalfe R and Chalk L, 1988. Anatomy of di-cotyledons. Oxford: Clarendon Press, 2nd ed. 1, 276. Riederer M and Schreiber L, 2001. Pro-tecting against water loss: analysis of the bar-rier properties of plant cuticles. Journal of ex-perimental botany, 52(363):2023-32.
15. Wallis TE, 1985. Textbook of Pharmacognosy. Published by SK Jain Webb MA, 1999. Cell-mediated crystallization of calcium oxalate in plants. The plant cell. 11(4):751- 61.
16. Woodward FI, 1998. Do plants really need stomata?. Journal of Experimental Bota-ny, 1:471- 80.
17. Kurman R. Blaustein's pathology of the fe-male genital tract. New York: Springer-Verlag, 1994.
18. Rubin SC, Sutton G. Ovarian can-cer. Philadelphia: Lippincott Williams & Wilkins, 2001.
19. Scully R, Sobin L. Histological typing of ovar-ian tumours, volume 9. New York: Springer Berlin, 1999.
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30. Silverberg SG. Histopathologic grading of ovarian carcinoma: a review and proposal. Int J Gynecol Pathol. 2000; 19: 7– 15.
31. Mayr D, Diebold J. Grading of ovarian carci-nomas. Int J Gynecol Pathol. 2000; 19: 348– 353.
32. Garcia A, Bussaglia E, Machin P, Matias-Guiu X, Prat J. Loss of heterozygosity on chromo-some 17q in epithelial ovarian tumors: associ-ation with carcinomas with serous differentia-tion. Int J Gynecol Pathol. 2000; 19: 152– 157.
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38. Alley, M. C., Scudiere, D. A., Monks, A., Czer-winski, M., Shoemaker, R. II., and Boyd, M. R. Validation of an automated microculture te-trazolium assay (MTA) to assess growth and drug sensitivity of human tumor cell lines. Proc. Am. Assoc. Cancer Res., 27: 389, 1986
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application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65: 55–63.
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41. https://www.mdpi.com/2072-6694/11/9/1357
42. https://www.mayoclinic.org/diseases-conditions/ovarian-cancer/symptoms-causes/syc-20375941#dialogId63367317
43. https://acsjournals.onlinelibrary.wiley.com/doi/10.1002/cncr.11345
44. https://www.cancer.gov/about-cancer/understanding/what-is-can-cer#:~:text=Cancer%20is%20a%20disease%20caused,are%20also%20called%20genetic%20changes
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15/06/2022
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M, S., B. H. M, D. lakshmi P, S. M, A. S, and S. rani Y. “A Detailed Study of Phytochemical and Invitro Anticancer Study on Euphorbia Heterophylla .l”. International Journal of Pharmacognosy and Chemistry, Vol. 3, no. 2, June 2022, pp. 91-106, doi:10.46796/ijpc.v3i2.326.
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