A REVIEW ON: RECENT ADVANTAGES IN HPLC: PRINCIPLES, METHOD DEVELOPMENT STRATEGIES – MODERN INNOVATIONS OF APPLICATIONS OF PHARMACEUTICAL ANALYSIS
Abstract
Chromatographic methods play a pivotal role in modern analytical chemistry, with High-Performance Thin Layer Chromatography (HPTLC) and High-Performance Liquid Chromatography (HPLC representing two of the most widely applied separation techniques.It provides accurate and reproducible assay results, ensuring that pharmaceutical products contain the appropriate drug concentration required for therapeutic effectiveness.The composition of the mobile phase is optimized to achieve adequate separation, proper peak shape, and reproducible retention time.International regulatory authorities such as the International Council for Harmonisation (ICH), United States Food and Drug Administration (FDA), European Medicines Agency (EMA), and World Health Organization (WHO) have established harmonized guidelines to ensure analytical method suitability, validation, and lifecycle management.
Keywords:
HPLC, Techniques, Mobile Phases, ADME, Chromatographic methods
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References
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4. Gaber Y, Törnvall U, Kumar MA, Amin MA, Hatti-Kaul R. HPLC-ESI-MS/MS characterization of novel peptide derivatives from enzymatically synthesized green chemicals. Green Chem. 2011;13(8):2021-2025.
5. Karmakar P, Chandra A. Combination Therapy in Management of Hypertension. 2021
6. Girase A, Mahale B, Dhankani A, Pawar S. Review On: RP-HPLC. 2024:21-7.
7. Wesolowski M, Leyk E. Coupled and Simultaneous Thermal Analysis Techniques in the Study of Pharmaceuticals. Pharmaceutics. 2023;15(6).
8. Kumar S, Basu M, Ghosh P, Pal U, Ghosh MK. COVID-19 therapeutics: Clinical application of repurposed drugs and futuristic strategies for target-based drug discovery. Genes Dis. 2023;10(4):1402-28.
9. Yabré M, Ferey L, Somé IT, Gaudin K. Greening Reversed-Phase Liquid Chromatography Methods Using Alternative Solvents for Pharmaceutical Analysis. Molecules. 2018;23(5).
10. Karavadi T, Challa B. Determination of Tadalafil in rat plasma by liquid chromatography tandam mass spectrometry: Application to a pharmacokinetic study. Der Pharmacia Lettre. 2012;4:1401-13.
11. Ahuja, S., & Dong, M. (2006). Handbook of Pharmaceutical Analysis by HPLC. Elsevier.
12. International Conference on Harmonisation (ICH). (2005). Validation of Analytical Procedures: Text and Methodology Q2(R1).
13. Swartz, M. E., & Krull, I. S. (2017). Analytical Method Development and Validation. CRC Press.
14. European Pharmacopoeia (Ph. Eur.). (2020). Chapter 2.2.46. Chromatographic Techniques.
15. Skoog, D. A., West, D. M., Holler, F. J., & Crouch, S. R. (2013). Fundamentals of Analytical Chemistry. Cengage Learning.
16. Snyder LR, Dolan JW. High-performance gradient elution: the practical application of the linear-solvent-strength model. John Wiley & Sons; 2007. p. 234-289.
17. Sandra P, Vanhoenacker G, David F, Sandra K, Pereira A. Green chromatography (Part 1): Introduction and liquid chromatography. LCGC Eur. 2010;23(5):242-259.
18. Reich E, Schibli A, DeBatt A. Validation of high-performance thin-layer chromatographic methods for the identification of botanicals in a cGMP environment. J AOAC Int. 2008;91(1):13-20
19. Dong MW, Guillarme D. UHPLC in life sciences. Royal Society of Chemistry; 2012. p. 145-198.
20. Vogeser M, Seger C. A decade of HPLC–MS/MS in the routine clinical laboratory—goals for further developments. Clin Biochem. 2008;41(9):649-662.
21. Yong Lin, Ranxin Shi, Xia Wang, Han-Ming Shen. Luteolin, a flavonoid with potentials for cancer prevention and therapy. Current Cancer Drug Targets 2008; 8(7):634-46.
22. Houghton PJ. Establishing identification criteria for botanicals. Drug Inf J 1998;32(2):461-69
23. Li L, Jiang H, Wu H, Zeng S. Simultaneous determination of luteolin and apigenin in dog plasma by RPHPLC. J Pharmaceut Biomed Anal 2005;37(3):615-20.
24. Chen Z, Kong S, Song F, Li L, Jiang H. Pharmacokinetic study of luteolin, apigenin, chrysoeriol and diosmetin after oral administration of Flos Chrysanthemi extract in rats. Fitoterapia 2012;83(8):1616-22.
25. Christou, C., Agapiou, A., & Kokkinofta, R. (2018). Use of FTIR spectroscopy and chemometrics for the classification of carobs origin. Journal of Advanced Research, 10, 1-8.
26. Wolfender, J. L. (2009). HPLC in natural product analysis: the detection issue. Planta medica, 75(07), 719- 734.
27. Schneider, A., Gerbi, V., & Redoglia, M. (1987). A rapid HPLC method for separation and determination of major organic acids in grape musts and wines. American Journal of Enology and Viticulture, 38(2), 151- 155.
28. Roy, P., Ahmed, M. A., & Kumer, A. (2019). An overview of hygiene practices and health risks related to street foods and drinking water from roadside restaurants of Khulna City of Bangladesh. Eurasian Journal of Environmental Research, 3(2), 47-55.
29. Vali SJ et al. Separation and Quantification of Octahydro-1h-Indole-2- Carboxilic Acid and Its Three Isomers by HPLC using Refractive Index Detector. J Chromate Separation Technique. 2012; 3:136.
Published
03/06/2026
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Badri, S., G, H. K., P, V., & U, S. (2026). A REVIEW ON: RECENT ADVANTAGES IN HPLC: PRINCIPLES, METHOD DEVELOPMENT STRATEGIES – MODERN INNOVATIONS OF APPLICATIONS OF PHARMACEUTICAL ANALYSIS. Journal of Innovations in Applied Pharmaceutical Science (JIAPS), 11(2), 33-39. https://doi.org/10.37022/jiaps.v11i2.899
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