Evaluation of antibacterial and antifungal activity of ocimum sanctum leaf extract

Madhavi Kumari M; Revathi Reddy Koyya

doi:10.37022/jiaps.v10i3.730

M.Madhavi Kumari Department of Pharmacology, Avanthi Institute of Pharmaceutical Sciences, Tagarapuvalasa, Vijayanagaram, A.P, India
Koyya Revathi Reddy Department of Pharmacology, Avanthi Institute of Pharmaceutical Sciences, Tagarapuvalasa, Vijayanagaram, A.P, India

DOI https://doi.org/10.37022/jiaps.v10i3.730

Abstract

The antimicrobial and antifungal properties of Ocimum sanctum leaf extract were the primary motivation for this investigation. The aim of this review is to summarize current antimicrobial research and identify the potential treatments for specific parasitic and bacterial infections. The ethnomedicinal herb Ocimum sanctum was studied for its microbiological activity, particularly in the development of potential antimicrobials against therapeutically significant bacterial and parasitic strains. Methanol was used to extract the active components from the leaves. The antibacterial effects of the extracts were assessed using the agar well diffusion method. A variety of microorganisms were tested, including two Gram-positive bacteria (Staphylococcus aureus and Streptococcus pyogenes), two Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and three fungal strains (Aspergillus niger, Aspergillus clavatus, and Candida albicans). The results indicated a significant inhibition of microbial growth, suggesting strong antimicrobial activity. Phytochemical analysis of the plant revealed a variety of secondary metabolites in close proximity, which may have acted synergistically to enhance the antimicrobial effect of Ocimum sanctum. These findings suggest that Ocimum sanctum holds promise as a source of bioactive compounds for further pharmaceutical research and development.

Keywords: Ocimum sanctum, Antimicrobial activity, Antifungal properties, Phytochemical analysis, Methanolic leaf extract, Pathogenic microorganisms

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