Simultaneous Estimation Of Curcumin And Piperine In Herbal Cough Syrup Using Hptlc

Abstract

Many of the traditional herbal formulations contain extracts of Piper longum and Curcuma longa, piperine and curcmin respectively, being active constituents of these two herbs. An attempt has been made to develop a simple, precise, rapid, and cost-effective high-performance thin-layer chromatographic (HPTLC) method for simultaneous estimation of these in a herbal cough formulation (Virak of Syrup). Pre-coated silica gel 60F-254 plates with chloroform: ethanol: ethyla cetate: acetic acid (9.5:0.5:1.0:0.2,v/v/v/v) as mobile phase were used in chromatographic determinations. The plates were scanned and the compounds were quantified at 360nm. The respective RF values of curcumin and piperine were 0.58 and 0.69. Under these experimental conditions linearity was observed between 200- 800ng/spot for curcumin and between 30- 90ng/spot for piperine and average recovery was 99.06% for curcumin and 95.13% for piperine.