Efficacy of 2, 6-diaryl-1-(2-(thiophene-2-yl) acetyl) piperidin-4-one oxime on wound infecting bacteria

Yasodha T; Vijayalakshmi M S; Hemachandran M

doi:10.46795/ijhcbs.v6i2.682

Yasodha T Professor, Department of Biotechnology, Madha Engineering College, Chennai, Tamilnadu, India
Vijayalakshmi M.S Assistant Professor, Department of Pharmaceutical Technology, Sri Muthukumaran Institute of Technology, Chennai, Tamilnadu, India
Hemachandran M Associate Professor, Department of Biomedical Engineering, Sri Muthukumaran Institute of Technology, Chennai, Tamilnadu, India

DOI https://doi.org/10.46795/ijhcbs.v6i2.682

Abstract

Background: Etiological classification of wounds may belong to acute or chronic.In these types tissue damage will lead to pathogenic infections and cause inflammation.Targeting fast healing of wounds by the control of pathogenic microflora and treatment with the heterocyclic compounds.

Objectives: The present study aimswith the objectives of synthesizedpiperidine oxime were profiled by 1H NMR and 13C NMR spectral experiments.It was investigated for its antibacterial potentiality against wound infecting bacteria.

Materials and Methods: The synthetic piperidine oxime was characterized by NMR. The concentrations of piperidine oxime were tested from 10µg/ml to 125 µg/mlagainst bacterial pathogens of wounds.Antibacterial activity was assessed by the zone of inhibition(ZOI). All the data were analysed by SPSS -IBM for the statistical significance.

RESULTS: NMR spectral studies revealed that the nitrogen site of 2,6-diphenyl piperidin-4-one ring exerted a minor change in the chemical shifts of the ring carbons and their attached protons. Furthermore, the benzylic protons at C-2 and C-6 position act as highly functionalized scaffolds.The zone of inhibition for Streptococcus pyogens is 26.5 mm,for Staphylococcus aureus is 25.9mm . ZOI was resulted at 27.2mm for Pseudomonas aeruginosa and the effect on Escherichia coli was recorded at 26.3 mm.

Conclusion: From the current research it had been proved that 100 µg of piperidin oxime could be sufficient to kill the wound pathogenic bacterial flora and piperidin oxime could be used an effective antibiotic as an alternative to amoxicillin.

Keywords: Piperidine 4-one oxime, NMR spectral study, wound bacterial isolates, antibacterial activity

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