Development and Evaluation of Natural Pain Relief Spray using polyherbal extracts

Abstract

Pain management is a critical aspect of healthcare, with growing interest in natural and herbal-based alternatives to synthetic analgesics. This study focuses on the development of a natural pain relief spray formulated using bioactive extracts from Mentha piperita (peppermint), Aloe vera, Arnica montana, and Calendula officinalis. The methodology involves the careful selection and authentication of raw materials, followed by optimized extraction techniques such as steam distillation, maceration, and freeze-drying to obtain key bioactive compounds. The extracted compounds were blended into a emulsion-based spray formulation using a combination of aqueous and ethanolic phases, ensuring uniform dispersion and stability. Natural stabilizers and preservatives were incorporated to enhance consistency and shelf-life, while pH adjustments were made to ensure skin compatibility. Standardization and quality control were conducted through UV-Vis spectroscopy, microbial load testing, viscosity measurement, and stability assessment under various environmental conditions. Furthermore, in-vitro anti-inflammatory and antioxidant assays were performed to evaluate the efficacy of the formulation. The developed pain relief spray demonstrated significant anti-inflammatory potential by inhibiting protein denaturation and stabilizing cell membranes, along with strong antioxidant activity. The final product was packaged in UV protected amber glass bottles to prevent photodegradation and ensure prolonged stability.  This study presents a scientifically validated, herbal-based pain relief spray as a safe and effective alternative for topical pain management