Validated RP-HPLC method for the simultaneous estimation of Proguanil and Atovaquone in pharmaceutical dosage form
Abstract
The aim of the study was to develop and validate a rapid, sensitive and accurate method for simultaneous estimation of proguanil and atovaquone in Pharmaceutical dosage forms by liquid chromatography.The chromatographic separation was achieved on Kromasil C18 (4.6 x 150 mm; 5 µm) at ambient temperature.The separation was achievedby employing a mobile phase consists of Phosphate buffer: acetonitrile (40:60 v/v). The flow rate was 1.0ml/ minute and ultra violet detector at 280 nm. The retention time for proguanil and atovaquone found to be 2.15 min and 2.48 min respectively. The proposed method was validated for selectivity, precision, linearity and accuracy. All the results obtained from various validation parameters were within the acceptable range. The method was found to be linear from concentrations of 20-120 µg/ml for proguanil and 50 - 300µg/ml for atovaquone.
Keywords:
Proguanil, Atovaquone, RP-HPLC
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References
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11. Allison B. Chambliss, Teresa L. Parsons, Mark A. Marzinke, An ultraperformance LC-MS/MS Method for the quantification of the antimalarial atovaquone in plasma, LC-MS/MS Quantification of ATQ 2017;1:400-409.
12. Satish GP, Ketan KN, Ajit MP, Uttam DP, Kiran VM, Determination of atovaquone in human plasma by LC-MS-MS and its application to a bioequivalence study, Chromatographia2009;70:947–951.
13. Viplava K, HarithaPavani V, Development and validation of stability-indicating RP-HPLC method for estimation of atovaquone, International Journal of Pharmaceutical and Clinical Research 2012;4:68-72.
14. Santosh V. Gandhi, Priyanka S. Ghone, Atul P. Chaudhari, HPTLC Method Development and Validation for Estimation of Atovaquone as a Bulk and in Pharmaceutical Dosage form, Pharmaceutical Resonance 2018;1:17- 20.
15. Varsha HC, Ajit AP, Kulkarni CG, Burade KB, Development & evaluation of spectrophotometric method forthe estimation of atovaquone in pharmaceutical dosage form, International Journal of Pharmaceutical Sciences and Research2013;4:3965-3970.
16. Veer VS, Badgujar MA, Kiran VM, Simultaneous determination of atovaquone and proguanil hydrochloride in tablet dosage form by high performance liquid chromatography,Research Journal of Pharmaceutical, Biological And Chemical Sciences 2012;3:377-383.
17. LakshmanaRao A, Prasanthi T, Fazeela T, Development and validation for simultaneous estimation of proguanil and atovaquone by using RP-HPLC, International Journal of Analytical Techniques 2017;3:1-10.
18. Lindegardh N, Blessborn D, Bergqvist Y, Simultaneous quantitation of the highly lipophilic atovaquone and hydrophilic strong basic proguanil and its metabolites using a new mixed-mode SPE approach and steep-gradient lc, Journal of Chromatographic Science 2005;43:1-8.
19. Naresh B, Swapnil JD, Raghavendra S, Prashant BM, New liquid chromatographic method for simultaneous quantification of atovaquone and proguanil with its active metabolite cycloguanil in human plasma,Indian Journal of Pharmaceutical Education and Research 2014;48:83-93.
20. Suraj S, Prasannakumar P, Sagarkumarmishra, Sabuj S, HPLC method development for simultaneous estimation of atovaquone and proguanil in tablet dosage form 2012;4:195-197.
21. Nitin SJ, Lalitha KG, Spectroscopic method development and validation for simultaneous estimation of atovaquone and aproguanil in tablet dosage form, World journal of Pharmacy and Pharmaceutical Sciences 2014;3:1986-1994.
2. Hussein Z, Eaves CJ, Hutchinson DB, Canfield CJ, Population pharmacokinetics of proguanil in patients with acute P.fulciparurnmalaria after combined therapy with atovaquone, Brazilian Journal of Clinical Pharmacology l996; 42: 589-597.
3. Helsby NA, Edwards G, Breckenridge AM, Ward SA, The multiple dose pharmacokinetics of proguanil, Brazilian Journal of Clinical Pharmacology1993; 35: 653-656.
4. Gemma L Nixon, Darren M Moss, Alison E Shone, David G. Lalloo, Nicholas Fisher, Paul M Neill, Stephen A Ward, Giancarlo A Biagini, Antimalarial pharmacology and therapeutics of atovaquone, Journal of Antimicrobial Chemotherapy 2013;68:977–985.
5. Satish GP, Ketan KN, Ajit MP, UttamDP, Kiran VM, Alternative LC–MS/MS Method for Simultaneous Determination of Proguanil, Its Active Metabolite in Human Plasma and Application to a Bioequivalence Study, Chromatographia 2009;70:1095-1102.
6. Nathalie L.Leveque, William N.Charman, Francis C.K.Chiu, Sensitive method for the quantitative determination of proguanil and its metabolites in rat blood and plasma by liquid chromatography–mass spectrometry, Journal of Chromatography B 2006; 830:314-321.
7. Adewuyi GO, Olubomehin O, Ayanniyi AW, High performance liquid chromatographic determination of proguanil after derivatisation with sodium benzoxazole-2-sulphonate, African Journal of Biotechnology 2010;9:900-905.
8. Nandini RP, Seema SS, Development and validation of new RP-HPLC method for determining impurity profiling in proguanil hydrochloride drug as well as it is tablet dosage form, Der PharmaChemica 2013;5:11-19.
9. Nandini RP, Seema SS, Development and validation of stability-indicating RP-HPLC method for estimation of proguanilHCl in tablet dosage form, Journal of Current Chemical and Pharmaceutical Sciences 2013;3:206-217.
10. Sanjay Gurule, DipanjanGoswami, Arshad H. Khuroo, TausifMonif, LC‐APCI mass spectrometric method development and validation for the determination of atovaquone in human plasma, Biomedical chromatography 2009;24:497-505.
11. Allison B. Chambliss, Teresa L. Parsons, Mark A. Marzinke, An ultraperformance LC-MS/MS Method for the quantification of the antimalarial atovaquone in plasma, LC-MS/MS Quantification of ATQ 2017;1:400-409.
12. Satish GP, Ketan KN, Ajit MP, Uttam DP, Kiran VM, Determination of atovaquone in human plasma by LC-MS-MS and its application to a bioequivalence study, Chromatographia2009;70:947–951.
13. Viplava K, HarithaPavani V, Development and validation of stability-indicating RP-HPLC method for estimation of atovaquone, International Journal of Pharmaceutical and Clinical Research 2012;4:68-72.
14. Santosh V. Gandhi, Priyanka S. Ghone, Atul P. Chaudhari, HPTLC Method Development and Validation for Estimation of Atovaquone as a Bulk and in Pharmaceutical Dosage form, Pharmaceutical Resonance 2018;1:17- 20.
15. Varsha HC, Ajit AP, Kulkarni CG, Burade KB, Development & evaluation of spectrophotometric method forthe estimation of atovaquone in pharmaceutical dosage form, International Journal of Pharmaceutical Sciences and Research2013;4:3965-3970.
16. Veer VS, Badgujar MA, Kiran VM, Simultaneous determination of atovaquone and proguanil hydrochloride in tablet dosage form by high performance liquid chromatography,Research Journal of Pharmaceutical, Biological And Chemical Sciences 2012;3:377-383.
17. LakshmanaRao A, Prasanthi T, Fazeela T, Development and validation for simultaneous estimation of proguanil and atovaquone by using RP-HPLC, International Journal of Analytical Techniques 2017;3:1-10.
18. Lindegardh N, Blessborn D, Bergqvist Y, Simultaneous quantitation of the highly lipophilic atovaquone and hydrophilic strong basic proguanil and its metabolites using a new mixed-mode SPE approach and steep-gradient lc, Journal of Chromatographic Science 2005;43:1-8.
19. Naresh B, Swapnil JD, Raghavendra S, Prashant BM, New liquid chromatographic method for simultaneous quantification of atovaquone and proguanil with its active metabolite cycloguanil in human plasma,Indian Journal of Pharmaceutical Education and Research 2014;48:83-93.
20. Suraj S, Prasannakumar P, Sagarkumarmishra, Sabuj S, HPLC method development for simultaneous estimation of atovaquone and proguanil in tablet dosage form 2012;4:195-197.
21. Nitin SJ, Lalitha KG, Spectroscopic method development and validation for simultaneous estimation of atovaquone and aproguanil in tablet dosage form, World journal of Pharmacy and Pharmaceutical Sciences 2014;3:1986-1994.
Published
25/10/2023
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How to Cite
U, M., and K. S. Nautiyal. “Validated RP-HPLC Method for the Simultaneous Estimation of Proguanil and Atovaquone in Pharmaceutical Dosage Form”. International Journal of Health Care and Biological Sciences, Vol. 4, no. 4, Oct. 2023, pp. 1-6, doi:10.46795/ijhcbs.v4i4.549.
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