Research Article In-vitro Anti-dermatophytic Activity of Different Medicinal Plant Extracts:

Sandeep Kaur; Prabhjot Kaur Gill

doi:10.46956/ijihd.v8i5.493

Sandeep Kaur Centre for Interdisciplinary Biomedical Research, Adesh University, Bathinda, Punjab, India
Prabhjot Kaur Gill Department of Genetics, Sri Guru Ram Das University of Health Sciences, Sri Amritsar,143501, Punjab, India

DOI https://doi.org/10.46956/ijihd.v8i5.493

Abstract

Background: Dermatophytosis is most common superficial skin infection and dermatophytes are fungi that invade within keratinized tissues; skin, hair and nails. In many studies reported that some medicinal plant are very useful to treat various skin disease included dermatophyte, because these medicinal plants are natural so have low cost, high availability, few side effects and valuable resources.

Objective: To evaluate the antifungal activity of Melaleuca alternifolia, Zingiber officinale, Allium sativum, Azadirachta indica, Citrus limonum, Curcuma longa, and Cocos nucifera against the different dermatophytes causing skin infections.

Methods: The Melaleuca alternifolia (tea tree oil), Azadirachta indica (neem oil), Cocos nucifera (coconut oil) and fresh juice of Citrus limonum (Lemon) and aqueous extracts of Zingiber officinale, Allium sativum and Curcuma longa were explored for antifungal susceptibility testing as per CLSI guidelines by agar well diffusion method.

Results: Anti-fungal potentials of the oils and aqueous extracts of different medicinal plants were tested against Trichophyton mentagrophytes, T. rubrum, T. tonsurans, T. verrucosum, Epidermophyton floccosum, Microsporum gypseum, M. canis. All the seven dermatophytes were susceptible to Melaleuca alternifolia, Zingiber officinale and Allium sativum, showed inhibitory zone ranges from 40±2.0mm to 45±2.0mm. Whereas, Azadirachta indica showed inhibitory zone ranges from 18±2.0 mm to 35±2.0mm for all the seven dermatophytes followed by Citrus limonum. On the contrary Curcuma longa and Cocos nucifera not effective against any tested dermatophytes.

Conclusion: The current research provides a scientific validation for the use of these medicinal plants in the treatment of dermatophytic infection and could be used in future for dermatophytic infection and other skin infection.

Keywords: medicinal plants, dermatophytes, keratinized tissue, antifungal activity, skin infection

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Author Biographies

Sandeep Kaur, Centre for Interdisciplinary Biomedical Research, Adesh University, Bathinda, Punjab, India

Centre for Interdisciplinary Biomedical Research, Adesh University, Bathinda, Punjab, India

Prabhjot Kaur Gill, Department of Genetics, Sri Guru Ram Das University of Health Sciences, Sri Amritsar,143501, Punjab, India

Department of Genetics, Sri Guru Ram Das University of Health Sciences, Sri Amritsar,143501, Punjab, India

References

1. Narain, U., Kumar, B.A. Onychomycosis: role of non-dermatophytes. Int. J. Adv. Med., 2016, 3(3), 643-647.
2. Ayatollahi Mousavi, S. A., Kazemi, A. In vitro and in vivo antidermatophytic activities of some Iranian medicinal plants. Med. Myco., 2015, 53(8), 852-859.
3. Matsuda, Y., Sugiura, K., Hashimoto, T., Ueda, A., Konno, Y., Tatsumi, Y. Efficacy coefficients determined using nail permeability and antifungal activity in keratin-containing media are useful for predicting clinical efficacies of topical drugs for onychomycosis. PLoS One, 2016, 11 (7).
4. Vandeputte, P., Ferrari, S., Coste, A. T. Antifungal resistance and new strategies to control fungal infections. Internat. J. Microbiol., 2012.
5. Bhatia, V. K., Sharma, P. C. Determination of minimum inhibitory concentrations of itraconazole, terbinafine and ketoconazole against dermatophyte species by broth microdilution method. Ind. J. Med. Microbiol., 2015, 33 (4): 533.
6. Piraccini, B. M., Alessandrini, A. Onychomycosis: a review. J. Fungi, 2015, 1(1), 30-43.
7. Sharma, R., Malik, A. Activity of natural products derived essential oils against dermatophytes. World J. Pharm. Pharm. Sci., 2015, 4, 1203-9.
8. Mahmoud D. A., Hassanein N. M., Youssef K. A. and Zeid A. Antifungal activity of different neem leaf extracts and the nimonol against some important human pathogens. Braz. J. Microbiol., 2011, 42 (3), 1007-1016
9. Dabas, Y., Xess, I., Singh, G., Pandey, M., Meena, S. Molecular identification and antifungal susceptibility patterns of clinical dermatophytes following CLSI and EUCAST guidelines. J. Fungi, 2017, 3(2), 17.
10. Lakshmipathy, D. T., Kannabiran, K. Review on dermatomycosis: pathogenesis and treatment. Natural Sci., 2010, 2(07), 726.
11. Sagar, K., Vidyasagar, G. M. Anti-dermatophytic activity of some traditionally used medicinal plants of North Karnataka Region. J. Appl. Pharm. Sci., 2013, 3(2).
12. Yu, D., Wang, J., Shao, X., Xu, F., Wang, H. Antifungal modes of action of tea tree oil and its two characteristic components against Botrytis cinerea. J. Appl. Microbiol., 2015, 119(5):1253-62.
13. Mao, Q.Q., Xu, X.Y., Cao, S.Y., Gan, R.Y., Corke, H., Beta, T., Li, H.B. Bioactive compounds and bioactivities of ginger (Zingiber officinale Roscoe). Foods, 2019, 8(6),185.
14. Leontiev, R., Hohaus, N., Jacob, C., Gruhlke, M.C., Slusarenko, A.J. A comparison of the antibacterial and antifungal activities of thiosulfinate analogues of allicin. Sci. rep., 2018, 8(1), 1-9.
15. Eja, M.E., Asikong, B.E., Abriba, C., Arikpo, G.E., Anwan, E.E., EnyiIdoh, K.H. A comparative assessment of the antimicrobial effects of garlic (Allium sativum) and antibiotics on diarrheagenic organisms. Southeast Asian J. Trop. Med. Public Health, 2007, 38, 343-348.
16. Karuppiah, P., Rajaram, S. Antibacterial effect of Allium sativum cloves and Zingiber officinale rhizomes against multiple-drug resistant clinical pathogens. Asian Pacific J. Trop. Biomed., 2012, 2(8), 597-601.
17. Rees, L.P., Minney, S.F., Plummer, N.T., Slater, J.H., Skyrme, D.A. A quantitative assessment of the antimicrobial activity of garlic (Allium sativum). World J. Microbiol. Biotechnol., 1993, 9, 303-307.
18. Weber, N.D., Anderson, D.O., North, J.A., Murray, B.K., Lawson, L.D., Hughes, B.G. In vitro virucidal effects of Allium sativum (garlic) extract and compounds. Planta Med., 1992, 58, 417-423.
19. Bokhari, F. M. Antifungal activity of some medicinal plants used in Jeddah, Saudi Arabia. Mycopath., 2009, 7(1), 51-57.
20. Dilig, H. A. M., Ilagan, M. F. A., Perey, J. B. N. Antifungal activity of some medicinal plants against selected species of dermatophytes. Cavite State University, 2016.
21. Adiguzel, A., Ozer, H., KiliC, H., CetiN, B. Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi. Czech J. Food Sci., 2007, 25(2), 81.
22. Aibinu, I., Adenipekun, T., Adelowotan, T., Ogunsanya, T., Odugbemi, T. Evaluation of the antimicrobial properties of different parts of Citrus aurantifolia (lime fruit) as used locally. Tradit. Complement. Altern. Med., 2007, 4(2), 185.
23. Shaikh, U., Abrar, M., Shaikh, M., Danish, A., Usama, K., Kalam, A. A Review: Household herbs have antifungal activity. World J. Pharm. Pharm. Sci., 2018, 7(9), 659-665.
24. Shahitha, S., Saranya, M., Poornima, K. Isolation and identification of dermatophytes from clinical samples and antidermatophytic activity of Lawsonia inermis (Henna plant). Int. J. Pharm. Chem. Sci., 2013. 2(2), 1014-17.
25. Imo, C., Za’aku, J. S. Medicinal Properties of Ginger and Garlic: A Review. Trends Biomed. Eng. Biosci, 2019, 18: 47-52.