A RAPID METHOD TO DETECT ANTIBIOTICS RESIDUCES IN HEN EGGS BY USING RP- HPLC

P.V. Suresh; Konda Ravi Kumar

P.V. Suresh Department of Pharmaceutical Analysis, Chalapathi Institute of Pharmaceutical Sciences, Lam, Guntur, Andhra Pradesh, India -522034
Konda Ravi Kumar Department of Pharmaceutical Chemistry, Hindu college of Pharmacy, Guntur, Andhra Pradesh, India -522002.

Abstract

The objective of this study is to quantify the antibiotics (Oxytetracycline & Enrofloxacin) residue levels in domestic and layers by RP-HPLC. This study was carried out on one hundred randomly collected domestic and layers hen egg samples (20 of each) to evaluate the antibiotic (oxytetracycline and enrofloxacin) residues level qualitatively by high performance liquid chromatography (HPLC). The separation was carried out using mobile phase composed of 0.1%formic acid: Acetonitrile (50:50, v/v) using C₁₈ (Hypersil ODS-BPS, 250 x 4.6mm; 5µ) at a flow rate of 1ml/min. The residues were quantified at detection wave length 350 nm. The obtained results revealed a detectable level of oxytetracycline and enrofloxacin residues which confirm widespread misuses of antibiotics in farms and lack of application of recommended withdrawal times. The boiling effect of broiler hen egg results shows the reduction in concentration of antibiotic residues. These findings recommended that restricted measures and harder regulations must be applied to prevent the misuse residues prior to marketing.

Keywords: Antibiotic, oxytetracycline, enrofloxacin, Maximum residue limit, HPLC

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